Abstract: The outline tests on the alignment of spur dikes in the flume with repeated tidal flow were carried out based on the alignment of the regulation structures in the north trough of the deep waterway in the Yangtze Estuary. Test results show that spur dikes aligned along a single bank in the deep water zone have more effects on the adjustment of the bed topography of the wide-shallow river than in the shallow water zone; alignment of spur dikes along both banks can effectively adjust the planimetric position of deep-water zone; and a reasonable widening rate of the regulation line in the estuary can readjust the variation of the average water depth along the river.