Abstract: Results of the flume experiments on non-uniform flow over a rough bed show that the vertical distribution of velocity on a channel section needs to be divided into two regions with different log velocity distribution equations. Velocity distribution on a rough bed is more non-uniform than that on a smooth bed, the turbulence intensity distribution of non-uniform flow becomes uniform with increase of the relative depth, and the regularities of the roughness coefficients of non-uniform flow on a rough bed are similar to those on a smooth bed.