The Wuxi Yundong surrounding water diversion test is divided into three phases: background period, dispatch period and recovery period. Through the long-series river network hydrodynamic-water quality multi-factor synchronous prototype observation, the hydrodynamic characteristics of main tributaries are obtained. The changes of water quality indicators at different control sections are mastered, and the sensitivity of different water quality indicators affected by the hydrodynamic process is analyzed. On this basis, in view of the diversion flow, ramp split ratio, river network flow rate, water level and water quality improvement range control threshold, the feasibility and existing problems of the main water diversion channel along Baiqu Port-Yanyu Port-Tuanshui River-Huancheng River-Grand Canal are demonstrated, and a regional refined river network hydrodynamic model is constructed. On the basis of model calibration and verification, several operation plans related to the cyanobacteria period are simulated, including the high-water level single-channel self-priming pumping, low-water level single-channel pumping and dual-channel self-priming pumping. The hydrodynamic and water quality effects of different ecological water replenishment schemes are selected to provide research methods and data support for the development of the Wuxi Yundong water scheme.